Part:BBa_K1919500:Design

K1321200+J04450 (Vitreoscilla hemoglobin + mRFP)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 477
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1293
  Illegal AgeI site found at 1405
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The Vitreoscilla haemoglobin gene sequence in iGEM database is not as the same as that in Genebank but it works.

Source

iGEM HQ.